Petection Antiseptic/Zone of Inhibition Testing
Testing was conducted in triplicate using a broth culture of Porphyromonas species. Using a sterile swab, the suspension was applied to surfaces of agar media supportive of anaerobic growth.Wells were created in the agar plates where a sample of the antiseptic gel, with a volume of .098mm3 or approximately 100μl, was injected. Following both 24 and 48 hours of incubation at 30 +/- 2°C, zones of inhibition, if any, were measured via microcalipers.
Test at 24 hours
|
Sample Identification |
Tests & Measurements (mm) |
Average |
||
|
1 |
2 |
3 |
||
|
PETECTION Antiseptic Gel - Test A |
5.8 |
6.0 |
6.0 |
5.9 |
|
PETECTION Antiseptic Gel - Test B |
6.0 |
6.0 |
6.2 |
6.1 |
|
PETECTION Antiseptic Gel - Test C |
6.0 |
5.8 |
5.9 |
5.9 |
|
Standard Liquid Rinse |
3.6 |
3.7 |
4.0 |
3.8 |
|
Untreated - Control (sterile water) |
0 |
0 |
0 |
0 |
|
Growth + Control |
8.2 |
9.3 |
8.3 |
8.6 |
Test at 48 hours
|
Sample Identification |
Tests & Measurements (mm) |
Average |
||
|
1 |
2 |
3 |
||
|
PETECTION Antiseptic Gel - Test A |
6.9 |
7.0 |
6.8 |
6.9 |
|
PETECTION Antiseptic Gel - Test B |
7.0 |
7.1 |
7.0 |
7.0 |
|
PETECTION Antiseptic Gel - Test C |
7.0 |
7.1 |
6.9 |
7.0 |
|
Standard Liquid Rinse |
3.6 |
7.1 |
3.7 |
4.8 |
|
Untreated - Control (sterile water) |
0 |
0 |
0 |
0 |
|
Growth + Control |
8.2 |
9.3 |
8.3 |
8.6 |
Zone of Inhibition at 24 hours against Porphyromonas species
Porphyromonas species were exposed to the test material for the proposed time intervals of 0, 30 seconds, 1, 5, 15, and 30 minutes. At the end of each exposure period, an appropriate neutralizing agent was used to quench the antimicrobial activity. Microorganisms before and after the treatment were counted to determine the antimicrobial activity of the test product, expressed in log 10 eduction format. Samples were plated on anerobic agar after each time point to establish the
survival counts.
Table 1: 24 hour (“young”) Porphyromonas species culture
|
Porphyromonas species |
0 |
30 sec |
1 min |
5 min |
15 min |
30 min |
|
8 million |
40 |
20 |
10 |
0 |
0 |
0 |
Table 2: 72 hour (“old”) Porphyromonas species culture
|
Porphyromonas species |
0 |
30 sec |
1 min |
5 min |
15 min |
30 min |
|
1 million 500 thousand |
6.0 x 103 |
1.1 x 103 |
2.0 x 102 |
0 |
0 |
0 |
Time Kill Test 24 hr “young” bacteria
Time Kill Test 72 hr “old” bacteria
Testing performed by an independent lab.
- Petection Antiseptic Gel